Fig 1: Effect of ST ethanolic extract administration on ultraviolet (UV)B-induced expression of matrix metalloproteinases (MMPs), pro-collagen, and tissue inhibitor of metalloproteinase 1 (TIMP1) following UVB-irradiation of skin tissue in hairless HR-1 mice. HR-1 mice were orally administered 100 or 200 mg/kg of ST ethanolic extract for 9 weeks. (A) Expression of MMPs (MMP1, 3, and 9) and pro-collagen A1 was detected by Western blot analysis and quantitative values of protein expression in HR-1 mice. All results were normalized to the normal control and calculated using ImageJ software. (B) The level of expression of TIMP1 was detected using an ELISA kit. Results are presented as the mean ± SEM from three independent experiments. # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. normal control; NC, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. UVB irradiation control; Con.
Supplier Page from Abcam for Mouse TIMP1 ELISA Kit